Generator

Part:BBa_K360111:Design

Designed by: Mariana Ruiz Velasco Leyva   Group: iGEM10_UNAM-Genomics_Mexico   (2010-10-10)

J23100 + RBS + LRE + BBa_B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 256
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 462
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 448


Design Notes

Construction is ligated into pSB1C3.


Source

LRE was first described by Gomi, K. and Kajiyama, N. from the Research and Development Division, Kikkoman Corporation.

References


  • Gomi, K. and Kajiyama, N. (2001) Oxyluciferin, a luminescence product of firefly luciferase, is enzymatically regenerated into luciferin. J. Biol. Chem. 276 (39), 36508-36513.